UbiQ Chains, all 8 natively-linked di-ubiquitin chains

UbiQ is the original and only manufacturer of all 8 natively-linked di-ubiquitin chains (Figure 1).1

Ubiquitin chains are built by forming a native isopeptide bond between the C-terminus of one ubiquitin and

one of the seven potential lysines (K6, K11, K27, K29, K33, K48, K63) or the N-terminus (linear chain) of the

preceding ubiquitin (Figure 2). All chain types have been identified in cells and the type of linkage in a

ubiquitin chain regulates distinct signals that affect physiological processes.2 Therefore access to all these

linkages is important in order to elucidate their biological roles and mode of action. UbiQChains offers a di-

ubiquitin explorer panel that makes it possible to test all linkages in a convenient and affordable way.3

UbiQ-Chains reagents can be used as a substrate for proteases that cleave the isopeptide linkage between

two ubiquitin protein known as deubiquitylases (DUBs). The reagents can also be used to investigate

mechanism of binding and recognition by proteins that contain ubiquitin binding motifs.

Figure 1.

Figure 2.

Features

• All linkages available

• Native isopeptide bond

• Native Ub sequence (i.e. no Lys-to-Arg mutations)

Applications

• DUB specificity

• Immunization

• Crystallography

References

1. El Oualid et al. Angew Chem Int Ed 2010, 49, 10149.

2. Dikic et al. Nature Reviews Molecular Cell Biology 2010, 10, 659.

3. (a) Licchesi et al. Nature Structural & Molecular Biology 2012, 19, 62. (b) Faesen et al. Chemistry &

Biology, 2011,18, 1550.

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